• Media type: E-Article; Text
  • Title: BeadNet: Deep learning-based bead detection and counting in low-resolution microscopy images
  • Contributor: Scherr, Tim [Author]; Streule, Karolin [Author]; Bartschat, Andreas [Author]; Böhland, Moritz [Author]; Stegmaier, Johannes [Author]; Reischl, Markus [Author]; Orian-Rousseau, Véronique [Author]; Mikut, Ralf [Author]
  • imprint: Oxford University Press, 2020-07-01
  • Published in: Bioinformatics, 36 (17), ISSN: 1367-4803, 1460-2059
  • Language: English
  • DOI: https://doi.org/10.5445/IR/1000120804; https://doi.org/10.1093/bioinformatics/btaa594
  • ISSN: 1367-4803; 1460-2059
  • Keywords: DATA processing & computer science
  • Origination:
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  • Description: Motivation An automated counting of beads is required for many high-throughput experiments such as studying mimicked bacterial invasion processes. However, state-of-the-art algorithms under- or overestimate the number of beads in low-resolution images. In addition, expert knowledge is needed to adjust parameters. Results In combination with our image labeling tool, BeadNet enables biologists to easily annotate and process their data reducing the expertise required in many existing image analysis pipelines. BeadNet outperforms state-of-the-art-algorithms in terms of missing, added and total amount of beads. Availability and implementation BeadNet (software, code and dataset) is available at https://bitbucket.org/t_scherr/beadnet. The image labeling tool is available at https://bitbucket.org/abartschat/imagelabelingtool.
  • Access State: Open Access