Metabolic architecture of the cereal grain and its relevance to maximize carbon use efficiency

Media type: Text; E-Article
Title: Metabolic architecture of the cereal grain and its relevance to maximize carbon use efficiency
Contributor: Rolletschek, Hardy [Author]; Grafahrend-Belau, Eva [Author]; Munz, Eberhard [Author]; Radchuk, Volodymyr V [Author]; Kartäusch, Ralf [Author]; Tschiersch, Henning [Author]; Melkus, Gerd [Author]; Schreiber, Falk [Author]; Jakob, Peter M [Author]; Borisjuk, Ljudmilla [Author]
Published: KOPS - The Institutional Repository of the University of Konstanz, 2015-09-22
Published in: Plant Physiology. 2015, 169(3), S. 1698-1713. ISSN 0032-0889. eISSN 1532-2548. Verfügbar unter: doi:10.1104/pp.15.00981
Language: English
DOI: https://doi.org/10.1104/pp.15.00981
ISBN: 1895030749
Origination:
Footnote: Diese Datenquelle enthält auch Bestandsnachweise, die nicht zu einem Volltext führen.
Description: Here, we have characterized the spatial heterogeneity of the cereal grain's metabolism and demonstrated how, by integrating a distinct set of metabolic strategies, the grain has evolved to become an almost perfect entity for carbon storage. In vivo imaging revealed light-induced cycles in assimilate supply toward the ear/grain of barley (Hordeum vulgare) and wheat (Triticum aestivum). In silico modeling predicted that, in the two grain storage organs (the endosperm and embryo), the light-induced shift in solute influx does cause adjustment in metabolic flux without changes in pathway utilization patterns. The enveloping, leaf-like pericarp, in contrast, shows major shifts in flux distribution (starch metabolism, photosynthesis, remobilization, and tricarboxylic acid cycle activity) allow to refix 79% of the CO2 released by the endosperm and embryo, allowing the grain to achieve an extraordinary high carbon conversion efficiency of 95%. Shading experiments demonstrated that ears are autonomously able to raise the influx of solutes in response to light, but with little effect on the steady-state levels of metabolites or transcripts or on the pattern of sugar distribution within the grain. The finding suggests the presence of a mechanism(s) able to ensure metabolic homeostasis in the face of short-term environmental fluctuation. The proposed multicomponent modeling approach is informative for predicting the metabolic effects of either an altered level of incident light or a momentary change in the supply of sucrose. It is therefore of potential value for assessing the impact of either breeding and/or biotechnological interventions aimed at increasing grain yield. ; published
Access State: Open Access

Search in field:

Recently searched for: