Footnote:
Diese Datenquelle enthält auch Bestandsnachweise, die nicht zu einem Volltext führen.
Description:
Potato (Solanum tuberosum L.) is one of the most important crops in the world. In addition to food and fodder, potato is also used for industrial purposes like production of adhesives, paper, and cosmetics. The vegetative growth phase of potato correlates with dry periods in spring and early summer, which are increased by climate change. Drought stress leads to morphological, physiological, and biochemical changes in the plant that have an extensive negative impact on the size and quality of the tubers. Since potato is a drought-sensitive species with its shallow root system, the interest in drought-tolerant cultivars is immense. Because ex vitro test systems are expensive and labor-intense and because additional parameters like other abiotic and biotic stressors influence the stress response, investigations in vitro are of great interest. Advantages of in vitro systems are the controlled light intensity, temperature, and supply of nutrients. Furthermore, pathogens can be excluded from the culture, and experiments require less space. Osmotic stress in vitro can be induced by adding an osmoticum, which lowers the osmotic potential in the culture medium. In the context of this work, an existing in vitro test system was optimised. For this purpose, the solid medium was replaced by liquid medium to enable that sorbitol can be added stepwise with increasing concentration. This resulted in two advantages: 1. the stress induction was gradual, and thus no osmotic shock was induced; 2. the plants were able to establish roots prior to the addition of the osmoticum, which allowed the stress that occurred by cutting the explants to be mitigated. This experiment showed that sorbitol was probably taken up by the roots and transported into the shoots, where it was detected by GC-MS. Furthermore, selected potato genotypes were investigated for their early drought stress response in open greenhouse and shelter experiments and their early osmotic stress response in vitro. For this purpose, candidate proteins for drought stress ...