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Roland, Laura [Author]; Grau, Michael [Author]; Matena, Julia [Author]; Teske, Michael [Author]; Gieseke, Matthias [Author]; Kampmann, Andreas [Author]; Beyerbach, Martin [Author]; Murua Escobar, Hugo [Author]; Haferkamp, Heinz [Author]; Gellrich, Nils-Claudius [Author]; Nolte, Ingo [Author]

Poly-epsilon-caprolactone Coated and Functionalized Porous Titanium and Magnesium Implants for Enhancing Angiogenesis in Critically Sized Bone Defects - [published Version]

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  • Media type: Text; E-Article
  • Title: Poly-epsilon-caprolactone Coated and Functionalized Porous Titanium and Magnesium Implants for Enhancing Angiogenesis in Critically Sized Bone Defects
  • Contributor: Roland, Laura [Author]; Grau, Michael [Author]; Matena, Julia [Author]; Teske, Michael [Author]; Gieseke, Matthias [Author]; Kampmann, Andreas [Author]; Beyerbach, Martin [Author]; Murua Escobar, Hugo [Author]; Haferkamp, Heinz [Author]; Gellrich, Nils-Claudius [Author]; Nolte, Ingo [Author]
  • imprint: Basel : MDPI AG, 2015
  • Published in: International Journal of Molecular Sciences 17 (2015), Nr. 1
  • Issue: published Version
  • Language: English
  • DOI: https://doi.org/10.15488/1587; https://doi.org/10.3390/ijms17010001
  • ISSN: 1422-0067
  • Keywords: Bone-Implant Interface/blood supply ; Hydroxybutyrates/adverse effects/pharmacology ; 0 (Polyesters) ; 0 (Hydroxybutyrates) ; I38ZP9992A (Magnesium) ; Titanium/adverse effects/pharmacology ; HMGB1 Protein/pharmacology ; Magnesium/adverse effects/pharmacology ; Cells ; D1JT611TNE (Titanium) ; Polyesters/adverse effects/pharmacology ; Vascular Endothelial Growth Factor A/pharmacology ; Mice ; Osteoblasts/drug effects/physiology ; 0 (Vascular Endothelial Growth Factor A) ; Physiologic ; 0 (HMGB1 Protein) ; Neovascularization ; Inbred C57BL ; Cell Line ; Porosity ; Cultured ; Animals ; Osseointegration
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  • Description: For healing of critically sized bone defects, biocompatible and angiogenesis supporting implants are favorable. Murine osteoblasts showed equal proliferation behavior on the polymers poly-epsilon-caprolactone (PCL) and poly-(3-hydroxybutyrate)/poly-(4-hydroxybutyrate) (P(3HB)/P(4HB)). As vitality was significantly better for PCL, it was chosen as a suitable coating material for further experiments. Titanium implants with 600 microm pore size were evaluated and found to be a good implant material for bone, as primary osteoblasts showed a vitality and proliferation onto the implants comparable to well bottom (WB). Pure porous titanium implants and PCL coated porous titanium implants were compared using Live Cell Imaging (LCI) with Green fluorescent protein (GFP)-osteoblasts. Cell count and cell covered area did not differ between the implants after seven days. To improve ingrowth of blood vessels into porous implants, proangiogenic factors like Vascular Endothelial Growth Factor (VEGF) and High Mobility Group Box 1 (HMGB1) were incorporated into PCL coated, porous titanium and magnesium implants. An angiogenesis assay was performed to establish an in vitro method for evaluating the impact of metallic implants on angiogenesis to reduce and refine animal experiments in future. Incorporated concentrations of proangiogenic factors were probably too low, as they did not lead to any effect. Magnesium implants did not yield evaluable results, as they led to pH increase and subsequent cell death. ; DFG/299/11-1
  • Access State: Open Access
  • Rights information: Attribution (CC BY)