• Media type: Text; E-Article
  • Title: Formation of Aliphatic and Aromatic α-Hydroxy Ketones by Zygosaccharomyces bisporus
  • Contributor: Neuser, Frauke [Author]; Zorn, Holger [Author]; Berger, Ralf Günter [Author]
  • Published: Berlin : De Gruyter, 2000
  • Published in: Zeitschrift für Naturforschung - Section C Journal of Biosciences 55 (2000), Nr. 7-8
  • Issue: published Version
  • Language: English
  • DOI: https://doi.org/10.15488/2422; https://doi.org/10.1515/znc-2000-7-814
  • ISSN: 0939-5075
  • Keywords: decarboxylation ; Pyruvate Decarboxylase ; Acyloin Formation ; chemical bond ; enzyme specificity ; stereospecificity ; enzyme substrate ; Zygosaccharomyces bisporus ; genetic strain ; wild relative ; yeast
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  • Description: The wild-type yeast strain Zygosaccharomyces bisporus CBS 702 produced a-hydroxy-ketones (acyloins) from amino acid precursors after transamination to the corresponding 2-oxo acids. The key enzyme of the subsequent decarboxylation and C-C bond forming reaction, pyruvate decarboxylase (PDC), was examined for its substrate- and stereo-specific-ity. A wide variety of saturated and unsaturated aliphatic and aromatic aldehydes was successfully converted to acyloins. 19 of the biotransformation products identified had not been reported as natural substances before. Product yields were strongly affected by substrate structure. © 2000, Verlag der Zeitschrift für Naturforschung. All rights reserved.
  • Access State: Open Access
  • Rights information: Attribution - Non Commercial - No Derivs (CC BY-NC-ND)