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Schmidt, Simone [Author]; Stahl, Frank [Author]; Mutz, Kai-Oliver [Author]; Scheper, Thomas [Author]; Hahn, Andreas [Author]; Schuchardt, Jan Philipp [Author]

Transcriptome-based identification of antioxidative gene expression after fish oil supplementation in normo- and dyslipidemic men - [published Version]

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  • Media type: E-Article; Text
  • Title: Transcriptome-based identification of antioxidative gene expression after fish oil supplementation in normo- and dyslipidemic men
  • Contributor: Schmidt, Simone [Author]; Stahl, Frank [Author]; Mutz, Kai-Oliver [Author]; Scheper, Thomas [Author]; Hahn, Andreas [Author]; Schuchardt, Jan Philipp [Author]
  • imprint: London : BioMed Central Ltd., 2012
  • Published in: Nutrition and Metabolism 9 (2012)
  • Issue: published Version
  • Language: English
  • DOI: https://doi.org/10.15488/678; https://doi.org/10.1186/1743-7075-9-45
  • ISSN: 1743-7075
  • Keywords: Omega-3 fatty acids ; Heme oxygenase ; heart protection ; clinical article ; nucleotide sequence ; drug effect ; cytochrome P450 1A2 ; unclassified drug ; human ; mitogen activated protein kinase ; transcriptome ; down regulation ; oxidative stress ; upregulation ; randomized controlled trial ; Antioxidative defence ; heme oxygenase 2 ; antioxidant activity ; erythrocyte membrane ; glutathione reductase ; Glutathione ; Oxylipines ; genetic transcription ; superoxide dismutase ; [...]
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  • Description: Background: The beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs), especially in dyslipidemic subjects with a high risk of cardiovascular disease, are widely described in the literature. A lot of effects of n-3 PUFAs and their oxidized metabolites are triggered by regulating the expression of genes. Currently, it is uncertain if the administration of n-3 PUFAs results in different expression changes of genes related to antioxidative mechanisms in normo- and dyslipidemic subjects, which may partly explain their cardioprotective effects. The aim of this study was to investigate the effects of n-3 PUFA supplementation on expression changes of genes involved in oxidative processes. Methods: Ten normo- and ten dyslipidemic men were supplemented for twelve weeks with fish oil capsules, providing 1.14 g docosahexaenoic acid and 1.56 g eicosapentaenoic acid. Gene expression levels were determined by whole genome microarray analysis and quantitative real-time polymerase chain reaction (qRT-PCR). Results: Using microarrays, we discovered an increased expression of antioxidative enzymes and a decreased expression of pro-oxidative and tissue enzymes, such as cytochrome P450 enzymes and matrix metalloproteinases, in both normo- and dyslipidemic men. An up-regulation of catalase and heme oxigenase 2 in both normo- and dyslipidemic subjects and an up-regulation of cytochrome P450 enzyme 1A2 only in dyslipidemic subjects could be observed by qRT-PCR analysis. Conclusions: Supplementation of normo- and dyslipidemic subjects with n-3 PUFAs changed the expression of genes related to oxidative processes, which may suggest antioxidative and potential cardioprotective effects of n-3 PUFAs. Further studies combining genetic and metabolic endpoints are needed to verify the regulative effects of n-3 PUFAs in antioxidative gene expression to better understand their beneficial effects in health and disease prevention. ; BMBF
  • Access State: Open Access