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Hsiao, I.-L. [Author]; Bierkandt, Frank [Author]; Reichardt, Ph. [Author]; Luch, A. [Author]; Huang, Y.-J. [Author]; Jakubowski, Norbert [Author]; Tentschert, J. [Author]; Haase, A. [Author]

Quantification and visualization of cellular uptake of TiO2 and Ag nanoparticles: comparison of different ICP-MS techniques

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  • Media type: E-Article
  • Title: Quantification and visualization of cellular uptake of TiO2 and Ag nanoparticles: comparison of different ICP-MS techniques
  • Contributor: Hsiao, I.-L. [Author]; Bierkandt, Frank [Author]; Reichardt, Ph. [Author]; Luch, A. [Author]; Huang, Y.-J. [Author]; Jakubowski, Norbert [Author]; Tentschert, J. [Author]; Haase, A. [Author]
  • imprint: BAM-Publica - Publikationsserver der Bundesanstalt für Materialforschung und -prüfung (BAM), 2016
  • Language: English
  • DOI: https://doi.org/10.1186/s12951-016-0203-z
  • Origination:
  • Footnote: Diese Datenquelle enthält auch Bestandsnachweise, die nicht zu einem Volltext führen.
  • Description: Safety assessment of nanoparticles (NPs) requires techniques that are suitable to quantify tissue and cellular uptake of NPs. The most commonly applied techniques for this purpose are based on inductively coupled plasma mass spectrometry (ICP-MS). Here we apply and compare three different ICP-MS methods to investigate the cellular uptake of TiO2 (diameter 7 or 20 nm, respectively) and Ag (diameter 50 or 75 nm, respectively) NPs into differentiated mouse neuroblastoma cells (Neuro-2a cells). Cells were incubated with different amounts of the NPs. Thereafter they were either directly analyzed by laser ablation ICP-MS (LA-ICP-MS) or were lysed and lysates were analyzed by ICP-MS and by single particle ICP-MS (SP-ICP-MS).
  • Access State: Open Access