Published in:doi:10.34734/FZJ-2023-05534 ; 2nd Workshop Carbon Allocation in Plants, Versailles, France, 2023-11-20 - 2023-11-21
Language:
English
DOI:
https://doi.org/10.34734/FZJ-2023-05534
Origination:
Footnote:
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Description:
Positron Emission Tomography (PET) is a potent tool in plant sciences to measure allocation and transport processes in vivo. Especially the radioisotope Carbon-11 is of major interest as it can be administered as 11CO2 to leaves and used as a tracer to monitor the flow of photoassimilates within complex 3D structures such as roots.However, in the past, PET studies on plants have primarily been conducted on clinical or preclinical PET scanners. These scanners have the drawback of being horizontally orientated and typically not optimized for a plant-friendly environment, including suboptimal temperature, humidity and light conditions. Studies conducted on plant dedicated scanners have often lacked a full characterization, quantitative image reconstruction (including attenuation correction of individual samples) and data analysis pipeline.In this poster we present the establishment of a complete routine Carbon-11 PET pipeline for plants. This pipeline begins with a custom PET facility for plants, which comprises the production and administration of 11CO2, gas exchange measurement, and a climate chamber with a fully characterized and calibrated plant-dedicated PET scanner, the phenoPET. It also includes workflow protocols to measure up to four plants per day and ensures that data from individual plants in measuring series can be reliably and quantitatively compared. Additionally, the application of an in-house developed compartmental model for long-distance transport allows us to quantify and analyze flow velocities of the tracer and exchange rates with other tissues along the transport pathways, including error calculation.