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Fasbender, Stefan [Author]; Allani, Sonja [Author]; Haas, Rainer [Author]; Wimmenauer, Christian [Author]; Cadeddu, Ron-Patrick [Author]; Raba, Katharina [Author]; Fischer, Johannes C. [Author]; Bulat, Bekir [Author]; Luysberg, Martina [Author]; Seidel, Claus A. M. [Author]; Heinzel, Thomas [Author]

Uptake dynamics of graphene quantum dots into primary human blood cells following in vitro exposure

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  • Media type: E-Article
  • Title: Uptake dynamics of graphene quantum dots into primary human blood cells following in vitro exposure
  • Contributor: Fasbender, Stefan [Author]; Allani, Sonja [Author]; Haas, Rainer [Author]; Wimmenauer, Christian [Author]; Cadeddu, Ron-Patrick [Author]; Raba, Katharina [Author]; Fischer, Johannes C. [Author]; Bulat, Bekir [Author]; Luysberg, Martina [Author]; Seidel, Claus A. M. [Author]; Heinzel, Thomas [Author]
  • Published: RSC Publishing, 2017
  • Published in: RSC Advances 7(20), 12208 - 12216 (2017). doi:10.1039/C6RA27829A
  • Language: English
  • DOI: https://doi.org/10.1039/C6RA27829A
  • ISSN: 2046-2069
  • Origination:
  • Footnote: Diese Datenquelle enthält auch Bestandsnachweise, die nicht zu einem Volltext führen.
  • Description: Human leukocytes obtained from samples of leukapheresis products of three healthy donors stimulated by granulocyte colony stimulating factor (G-CSF) were exposed to graphene quantum dots. A time- and concentration dependent uptake was observed with a significantly greater uptake into monocytes and granulocytes in comparison to lymphocytes, suggesting a better incorporation ability of cells with phagocytotic properties. The uptake rates also correlate with the cell membrane area. Looking at the different lymphoid subsets a greater uptake was found into CD19+ B-, CD56+ natural killer cells and CD34+ hematopoietic stem cells (HSC) in comparison to CD4+ T- and CD8+ T cells. Independent of the cell type studied, the observed uptake dynamics is consistent with a diffusion-driven process, which allows the determination of cell-specific membrane permeabilities for the graphene quantum dots. The toxicity of the quantum dots is relatively low resulting in a 90% viability of the entire leukocyte population after 36 hours of exposure to GQDs at a concentration of 500 μg ml−1.
  • Access State: Open Access