Development and application of Crystal Digital PCR-based single pollen nucleus genotyping to measure meiotic recombination rates in barley (Hordeum vulgare) in high- throughput

Media type: E-Book

Title: Development and application of Crystal Digital PCR-based single pollen nucleus genotyping to measure meiotic recombination rates in barley (Hordeum vulgare) in high- throughput

Contributor: Ahn, Yun-Jae [Author]; Houben, Andreas [Other]; Sanchez-Moran, Eugenio [Other]

Published: Halle (Saale): Universitäts- und Landesbibliothek Sachsen-Anhalt, 2022

Extent: Online-Ressource

Language: English

DOI: 10.25673/85943

Identifier:

Keywords: Pollen ; Barley ; Delivery of c hemical compounds: • Compounds were selected based on their inhibitory effects on post translational modifications according to previous studies • Pre screening of compounds for their inhibitory effect on root growth & mitotic cell cycle in barle y (Zebularine ; Tricho statin A ; BIX 01294) • Compounds were injected with needles to be delivered into meiotic cells (Ahn et al .) Single pollen genotyping: • Single pollen nucleus genotyping via digital PCR without WGA was established (Ahn et al . 2021) • Plants with different genome & nucleus size are compatible with the established method • Differences in the recombination rates were measured for i) plants grown under different growth conditions ii) different tillers of single plants iii) chemical treated vs. untreated plants • Some of the Zebularine injected plants showed a two fold increase in the recombination rate of the centromeric interval ; Gerste ; Crossover ; Crystal Digital PCRTM ; Hordeum vulgare ; Meiose ; meiotische Rekombination ; Genotypisierung eines Pollenkerns ; Einzelzellanalyse ; barley ; crossover ; meiosis ; meiotic recombination ; single pollen nucleus genotyping ; single-cell analysis

Origination:

University thesis: Dissertation, Halle (Saale), Martin-Luther-Universität Halle-Wittenberg, 2022

Footnote:

Access State: Open Access

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