Liu, Juan
[Author];
Tahir Khan, Muhammad
[Author];
Tang, Yong
[Author];
Franke, Heike
[Author];
Illes, Peter
[Author]
Inflammatory Cytokines Facilitate the Sensitivity of P2X7 Receptors Toward Extracellular ATP at Neural Progenitor Cells of the Rodent Hippocampal Subgranular Zone
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Media type:
E-Article
Title:
Inflammatory Cytokines Facilitate the Sensitivity of P2X7 Receptors Toward Extracellular ATP at Neural Progenitor Cells of the Rodent Hippocampal Subgranular Zone
Contributor:
Liu, Juan
[Author];
Tahir Khan, Muhammad
[Author];
Tang, Yong
[Author];
Franke, Heike
[Author];
Illes, Peter
[Author]
imprint:
Basel: MDPI, [2023]
Published in:Neuroglia ; 1,1 (2018), Seite 258-270
Description:
Organotypic hippocampal slice cultures were used to model the effects ofneuroinflammatory conditions following an epileptic state on functional P2X7 receptors (Rs) ofsubgranular zone (SGZ) neural progenitor cells (NPCs). The compound, 4-aminopyridine (4-AP),is known to cause pathological firing of neurons, consequently facilitating the release of varioustransmitter substances including ATP. Lipopolysaccharide (LPS) and interleukin-1(IL-1) bothpotentiated the dibenzoyl-ATP (Bz-ATP)-induced current amplitudes in NPCs, although via differentmechanisms. Whereas LPS acted via promoting ATP release, IL-1 acted via its own receptorto directly influence P2X7Rs. Thus, the effect of LPS was inhibited by the ecto-ATPase inhibitor,apyrase, but not by the IL-1 antagonist, interleukin-1RA (IL-1RA); by contrast, the effect of IL-1was inhibited by IL-1RA, but not by apyrase. Eventually, incubation with 4-AP upregulated thenumber of nestin/glial fibrillary acidic protein/P2X7R immunoreactive cells and their appropriatestaining intensity, suggesting increased synthesis of P2X7Rs at NPCs. In conclusion, inflammatorycytokines accumulating after epilepsy-like neuronal firing may facilitate the effect of endogenousATP at P2X7Rs of NPCs, thereby probably promoting necrosis/apoptosis and subsequent cell death.