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Sela, Ilan [Author]; Yaskolka Meir, Anat [Author]; Brandis, Alexander [Author]; Krajmalnik-Brown, Rosa [Author]; Zeibich, Lydia [Author]; Chang, Debbie [Author]; Dirks, Blake [Author]; Tsaban, Gal [Author]; Kaplan, Alon [Author]; Rinott, Ehut [Author]; Zelicha, Hila [Author]; Arinos, Shira [Author]; Ceglarek, Uta [Author]; Isermann, Berend [Author]; Lapidot, Miri [Author]; Green, Ralph [Author]; Shai, Iris [Author]

Wolffia globosa–Mankai Plant-Based Protein Contains Bioactive Vitamin B12 and Is Well Absorbed in Humans

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  • Media type: E-Article
  • Title: Wolffia globosa–Mankai Plant-Based Protein Contains Bioactive Vitamin B12 and Is Well Absorbed in Humans
  • Contributor: Sela, Ilan [Author]; Yaskolka Meir, Anat [Author]; Brandis, Alexander [Author]; Krajmalnik-Brown, Rosa [Author]; Zeibich, Lydia [Author]; Chang, Debbie [Author]; Dirks, Blake [Author]; Tsaban, Gal [Author]; Kaplan, Alon [Author]; Rinott, Ehut [Author]; Zelicha, Hila [Author]; Arinos, Shira [Author]; Ceglarek, Uta [Author]; Isermann, Berend [Author]; Lapidot, Miri [Author]; Green, Ralph [Author]; Shai, Iris [Author]
  • Published: Basel: MDPI, [2023]
  • Published in: Nutrients ; 12,10, (2020)
  • Language: English
  • Keywords: flexitarians ; Wolffia globosa ; vitamin B12 ; weight loss ; sustainability ; plant-based nutrition
  • Origination:
  • Footnote:
  • Description: Background: Rare plants that contain corrinoid compounds mostly comprise cobalamin analogues, which may compete with cobalamin (vitamin B12 (B12)) metabolism. We examined the presence of B12 in a cultivated strain of an aquatic plant: Wolffia globosa (Mankai), and predicted functional pathways using gut-bioreactor, and the effects of long-term Mankai consumption as a partial meat substitute, on serum B12 concentrations. Methods: We used microbiological assay, liquid-chromatography/electrospray-ionization-tandem-mass-spectrometry (LC-MS/MS), and anoxic bioreactors for the B12 experiments. We explored the effect of a green Mediterranean/low-meat diet, containing 100 g of frozen Mankai shake/day, on serum B12 levels during the 18-month DIRECT-PLUS (ID:NCT03020186) weight-loss trial, compared with control and Mediterranean diet groups. Results: The B12 content of Mankai was consistent at different seasons (p = 0.76). Several cobalamin congeners (Hydroxocobalamin(OH-B12); 5-deoxyadenosylcobalamin(Ado-B12); methylcobalamin(Me-B12); cyanocobalamin(CN-B12)) were identified in Mankai extracts, whereas no pseudo B12 was detected. A higher abundance of 16S-rRNA gene amplicon sequences associated with a genome containing a KEGG ortholog involved in microbial B12 metabolism were observed, compared with control bioreactors that lacked Mankai. Following the DIRECT-PLUS intervention (n = 294 participants; retention-rate = 89%; baseline B12 = 420.5 ± 187.8 pg/mL), serum B12 increased by 5.2% in control, 9.9% in Mediterranean, and 15.4% in Mankai-containing green Mediterranean/low-meat diets (p = 0.025 between extreme groups). Conclusions: Mankai plant contains bioactive B12 compounds and could serve as a B12 plant-based food source.
  • Access State: Open Access
  • Rights information: Attribution (CC BY)