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Media type:
E-Article
Title:
Transcriptome Analyses of Adipose Tissue Samples Identify EGFL6 as a Candidate Gene Involved in Obesity-Related Adipose Tissue Dysfunction in Children
Contributor:
Landgraf, Kathrin
[Author];
Kühnapfel, Andreas
[Author];
Schlanstein, Maria
[Author];
Biemann, Ronald
[Author];
Isermann, Berend
[Author];
Kempf, Elena
[Author];
Kirsten, Holger
[Author];
Scholz, Markus
[Author];
Körner, Antje
[Author]
Published:
Basel: MDPI, [2024]
Published in:International Journal of Molecular Sciences ; 23, (2022)
Description:
Obesity develops early in childhood and is accompanied by early signs of adipose tissue(AT) dysfunction and metabolic disease in children. In order to analyse the molecular processes duringobesity-related AT accumulation in children, we investigated genome-wide expression profiles in ATsamples, isolated adipocytes, and stromal vascular fraction (SVF) cells and assessed their relation toobesity as well as biological and functional AT parameters. We detected alterations in gene expressionassociated with obesity and related parameters, i.e., BMI SDS, adipocyte size, macrophage infiltration,adiponectin, and/or leptin. While differential gene expression in AT and adipocytes shared anenrichment in metabolic pathways and pathways related to extracellular structural organisation, SVFcells showed an overrepresentation in inflammatory pathways. In adipocytes, we found the strongestpositive association for epidermal growth factor-like protein 6 (EGFL6) with adipocyte hypertrophy.EGFL6 was also upregulated during in vitro adipocyte differentiation. In children, EGFL6 expressionwas positively correlated to parameters of AT dysfunction and metabolic disease such as macrophageinfiltration into AT, hs-CRP, leptin levels, and HOMA-IR. In conclusion, we provide evidence forearly alterations in AT gene expression related to AT dysfunction in children and identified EGFL6 aspotentially being involved in processes underlying the pathogenesis of metabolic disease.