Seidel, Elisabeth Christiane
[Author];
Birkemeyer, Claudia
[Author];
Baran-Schmidt, Rainer
[Author];
Meixensberger, Jürgen
[Author];
Oppermann, Henry
[Author];
Gaunitz, Frank
[Author]
Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazole-Containing Compounds
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Media type:
E-Article
Title:
Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazole-Containing Compounds
Contributor:
Seidel, Elisabeth Christiane
[Author];
Birkemeyer, Claudia
[Author];
Baran-Schmidt, Rainer
[Author];
Meixensberger, Jürgen
[Author];
Oppermann, Henry
[Author];
Gaunitz, Frank
[Author]
Footnote:
Hinweis: Link zur Erstveröffentlichung URL: https://doi.org/10.3390/ijms23105834
Description:
The naturally occurring dipeptide carnosine (-alanyl-L-histidine) specifically attenuates
tumor growth. Here, we ask whether other small imidazole-containing compounds also affect
the viability of tumor cells without affecting non-malignant cells and whether the formation of
histamine is involved. Patient-derived fibroblasts and glioblastoma cells were treated with carnosine,
L-alanyl-L-histidine (LA-LH), -alanyl-L-alanine, L-histidine, histamine, imidazole, -alanine, and
L-alanine. Cell viability was assessed by cell-based assays and microscopy. The intracellular release of
L-histidine and formation of histamine was investigated by high-performance liquid chromatography
coupled to mass spectrometry. Carnosine and LA-LH inhibited tumor cell growth with minor
effects on fibroblasts, and L-histidine, histamine, and imidazole affected viability in both cell types.
Compounds without the imidazole moiety did not diminish viability. In the presence of LA-LH but
not in the presence of carnosine, a significant rise in intracellular amounts of histidine was detected
in all cells. The formation of histamine was not detectable in the presence of carnosine, LA-LH, or
histidine. In conclusion, the imidazole moiety of carnosine contributes to its anti-neoplastic effect,
which is also seen in the presence of histidine and LA-LH. Despite the fact that histamine has a strong
effect on cell viability, the formation of histamine is not responsible for the effects on the cell viability
of carnosine, LA-LH, and histidine.