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Jensen Pedersen, Kristian [Author]; Haange, Sven-Bastiaan [Author]; Žížalová, Katerina [Author]; Viehof, Alina [Author]; Clavel, Thomas [Author]; Lenicek, Martin [Author]; Engelmann, Beatrice [Author]; Wick, Lukas Y. [Author]; Schaap, Frank G. [Author]; Jehmlich, Nico [Author]; Rolle-Kampczyk, Ulrike [Author]; von Bergen, Martin [Author]

Eggerthella lenta DSM 2243 Alleviates Bile Acid Stress Response in Clostridium ramosum and Anaerostipes caccae by Transformation of Bile Acids

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  • Media type: E-Article
  • Title: Eggerthella lenta DSM 2243 Alleviates Bile Acid Stress Response in Clostridium ramosum and Anaerostipes caccae by Transformation of Bile Acids
  • Contributor: Jensen Pedersen, Kristian [Author]; Haange, Sven-Bastiaan [Author]; Žížalová, Katerina [Author]; Viehof, Alina [Author]; Clavel, Thomas [Author]; Lenicek, Martin [Author]; Engelmann, Beatrice [Author]; Wick, Lukas Y. [Author]; Schaap, Frank G. [Author]; Jehmlich, Nico [Author]; Rolle-Kampczyk, Ulrike [Author]; von Bergen, Martin [Author]
  • Published: Basel: MDPI, [2024]
  • Published in: Microorganisms ; 10,10 (2022), Seite 1-22
  • Language: English
  • DOI: 10.3390/microorganisms10102025
  • Keywords: hydroysteroid dehydrogenase ; metabolomics ; bile acids ; eggerthella lenta ; metaproteomics ; gut microbiome interaction
  • Origination:
  • Footnote:
  • Description: Bile acids are crucial for the uptake of dietary lipids and can shape the gut-microbiomecomposition. This latter function is associated with the toxicity of bile acids and can be modulatedby bile acid modifying bacteria such as Eggerthella lenta, but the molecular details of the interactionof bacteria depending on bile acid modifications are not well understood. In order to unravel themolecular response to bile acids and their metabolites, we cultivated eight strains from a humanintestinal microbiome model alone and in co-culture with Eggerthella lenta in the presence of cholicacid (CA) and deoxycholic acid (DCA). We observed growth inhibition of particularly gram-positivestrains such as Clostridium ramosum and the gram-variable Anaerostipes cacae by CA and DCAstress. C. ramosum was alleviated through co-culturing with Eggerthella lenta. We approached effectson the membrane by zeta potential and genotoxic and metabolic effects by (meta)proteomic andmetabolomic analyses. Co-culturing with Eggerthella lenta decreased both CA and DCA by theformation of oxidized and epimerized bile acids. Eggerthella lenta also produces microbial bile saltconjugates in a co-cultured species-specific manner. This study highlights how the interaction withother bacteria can influence the functionality of bacteria.
  • Access State: Open Access
  • Rights information: Attribution (CC BY)