Stowell, Michael H. B.;
Soltis, S. Michael;
Kisker, Caroline;
Peters, John W.;
Schindelin, Hermann;
Rees, D. C.;
Cascio, Duilio;
Beamer, Lesa;
Hart, John;
Whitby, Frank G.
A simple pressure cell and delivery system for the preparation of Xe derivatives for protein crystallography
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Media type:
E-Article
Title:
A simple pressure cell and delivery system for the preparation of Xe derivatives for protein crystallography
Contributor:
Stowell, Michael H. B.;
Soltis, S. Michael;
Kisker, Caroline;
Peters, John W.;
Schindelin, Hermann;
Rees, D. C.;
Cascio, Duilio;
Beamer, Lesa;
Hart, John;
Whitby, Frank G.
Description:
<jats:p>We have developed a simple device for preparing Xe derivatives under moderate gaseous pressure (1–100 atm). The device employs a Cajon ultra-torr fitting to ensure a gas-tight seal around a standard x-ray capillary. As such, the cell can accommodate standard x-ray capillaries up to 1.5 mm in diameter without any modification. The device is straightforward to utilize, and samples can be mounted and pressurized in a matter of seconds. In addition, a simple and safe purging and pressurization system has been designed and constructed for the use at beamline 7-1 at the Stanford Synchrotron Radiation Laboratory (SSRL). We describe the construction of both the pressure cell and the delivery system and present results on the cells use in the preparation of xenon derivatives.</jats:p>