Description:
<jats:p>Evaporation-driven internal flows within a sessile droplet can transport microorganisms close to the leaf surface and facilitate their infiltration into the available openings, such as stomata. Here, using microfabricated surfaces out of polydimethylsiloxane, the sole effects of evaporation of sessile droplets in contamination of plant leaves was studied. These surfaces were patterned with stomata, trichomes, and grooves that are common surface microstructures on plant leaves. Evaporation of sessile droplets, containing bacterial suspensions, on real leaves and fabricated surfaces was studied using confocal microscopy. To provide insight about the effects of leaf hydrophobicity and surface roughness on the bacterial retention and infiltration, variations of contact angle of sessile droplets at these surfaces were measured during evaporation. The results showed that evaporation-driven flow transported bacteria close to the surface of spinach leaves and fabricated surfaces, leading to distinct infiltration into the stomata. Larger size and wider spacing of the micropores, and a more hydrophilic surface, led bacteria to spread more at the droplet base area and infiltrate into more stomata. Evaporation-driven movement of contact line, which can sweep bacteria over the leaf surface, was shown to lead to bacterial infiltration into the stomatal pores. Findings should help improve microbial safety of leafy greens.</jats:p>