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Media type:
E-Article
Title:
Visualization of the type III secretion sorting platform of Shigella flexneri
Contributor:
Hu, Bo;
Morado, Dustin R.;
Margolin, William;
Rohde, John R.;
Arizmendi, Olivia;
Picking, Wendy L.;
Picking, William D.;
Liu, Jun
Published:
Proceedings of the National Academy of Sciences, 2015
Published in:
Proceedings of the National Academy of Sciences, 112 (2015) 4, Seite 1047-1052
Language:
English
DOI:
10.1073/pnas.1411610112
ISSN:
0027-8424;
1091-6490
Origination:
Footnote:
Description:
Significance Many infectious bacteria such as Shigella and Salmonella use type III secretion machines, also called injectisomes, to transfer virulence proteins into eukaryotic host cells. A cytoplasmic sorting platform is required for effector selection and assembly of the needle but has not been visualized in any bacteria. We combine advanced imaging and genetic techniques to visualize the frozen-hydrated diarrheal pathogen Shigella flexneri and reveal the intact type III secretion machine and its interaction with a host cell for the first time to our knowledge. The structures characterized herein provide new insights into the mechanisms underlying type III secretion and pathogenesis and also highlight the major distinctions from the evolutionarily related bacterial flagellum.