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Media type:
E-Article
Title:
CryoEM and mutagenesis reveal that the smallest capsid protein cements and stabilizes Kaposi's sarcoma-associated herpesvirus capsid
Contributor:
Dai, Xinghong;
Gong, Danyang;
Xiao, Yuchen;
Wu, Ting-Ting;
Sun, Ren;
Zhou, Z. Hong
Published:
Proceedings of the National Academy of Sciences, 2015
Published in:
Proceedings of the National Academy of Sciences, 112 (2015) 7
Language:
English
DOI:
10.1073/pnas.1420317112
ISSN:
0027-8424;
1091-6490
Origination:
Footnote:
Description:
<jats:title>Significance</jats:title>
<jats:p>Kaposi's sarcoma-associated herpesvirus (KSHV) and EBV are cancer-causing human herpesviruses. Their smallest capsid proteins (SCPs) were shown to be required for capsid assembly and are potential drug targets for curbing viral infections, but how they work is unclear. By cryoEM and genetic engineering, we determine the structures of KSHV capsids bearing full-length or truncated SCPs and localize regions of SCP that are important for capsid assembly. We show that a long kinked helix of SCP cross-links neighboring subunits of the major capsid protein of hexons to stabilize the capsid. Our results explain how SCP, acting like a cementing protein found in bacterial viruses, facilitates tumor herpesvirus capsid assembly and viral maturation.</jats:p>