imprint:
Proceedings of the National Academy of Sciences, 2015
Published in:Proceedings of the National Academy of Sciences
Language:
English
DOI:
10.1073/pnas.1514209112
ISSN:
0027-8424;
1091-6490
Origination:
Footnote:
Description:
<jats:title>Significance</jats:title>
<jats:p>
We introduce a two-photon imaging method with improved depth penetration for the recording of neuronal activity with single-cell resolution in the intact brain of living animals. This method relies on the use of the fluorometric Ca
<jats:sup>2+</jats:sup>
-sensitive dye Cal-590, which is effectively excited by infrared light (1,050 nm). By combining population Ca
<jats:sup>2+</jats:sup>
imaging and electrical recordings in vivo, we demonstrate that neuronal activity can be monitored in all six layers of the mouse cortex. In combination with spectrally different Ca
<jats:sup>2+</jats:sup>
indicators, Cal-590 can be used for the simultaneous imaging of neuronal activity in distinct neuronal populations.
</jats:p>