Description:
<jats:title>Significance</jats:title>
<jats:p>The precision of localization-based superresolution microscopy techniques fundamentally relies on the point-spread function of the optical system and the number of photons one can collect. Here, we report that by using a high-numerical-aperture objective lens and a custom cryogenic stage, we are able to increase photon yield by 2–3-fold over room temperature, thereby achieving more precise superresolution reconstructions of complex subcellular structures.</jats:p>