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Media type:
E-Article
Title:
MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution
Contributor:
Eilers, Yvan;
Ta, Haisen;
Gwosch, Klaus C.;
Balzarotti, Francisco;
Hell, Stefan W.
imprint:
Proceedings of the National Academy of Sciences, 2018
Published in:Proceedings of the National Academy of Sciences
Language:
English
DOI:
10.1073/pnas.1801672115
ISSN:
0027-8424;
1091-6490
Origination:
Footnote:
Description:
<jats:title>Significance</jats:title>
<jats:p>Popular localization of single molecules through calculating the centroid of the diffraction pattern produced by molecular fluorescence on a camera is typically limited to spatiotemporal resolutions of >10 nm per >10 milliseconds. By requiring at least 10–100 times fewer detected photons and being free of bias due to molecular orientation, the localization concept called MINFLUX propels molecular tracking to the hitherto-unachievable regime of single-digit nanometer precision within substantially less than a millisecond. Our experiments herald the feasibility to detect molecular interactions and conformational changes at microsecond timescales.</jats:p>