You can manage bookmarks using lists, please log in to your user account for this.
Media type:
E-Article
Title:
Preferential binding of ATR protein to UV-damaged DNA
Contributor:
Ünsal-Kaçmaz, Keziban;
Makhov, Alexander M.;
Griffith, Jack D.;
Sancar, Aziz
Published:
Proceedings of the National Academy of Sciences, 2002
Published in:
Proceedings of the National Academy of Sciences, 99 (2002) 10, Seite 6673-6678
Language:
English
DOI:
10.1073/pnas.102167799
ISSN:
1091-6490;
0027-8424
Origination:
Footnote:
Description:
<jats:p>The ATR protein is a member of the phosphoinositide 3-kinase-related kinase family and plays an important role in UV-induced DNA damage checkpoint response. Its role as a signal transducer in cell cycle checkpoint is well established, but it is currently unclear whether ATR functions as a damage sensor as well. Here we have purified the ATR protein and investigated its interaction with DNA by using biochemical analysis and electron microscopy. We find that ATR is a DNA-binding protein with higher affinity to UV-damaged than undamaged DNA. In addition, damaged DNA stimulates the kinase activity of ATR to a significantly higher level than undamaged DNA. Our data suggest that ATR may function as an initial sensor in the DNA damage checkpoint response.</jats:p>