Barczak, Amy K.;
Gomez, James E.;
Kaufmann, Benjamin B.;
Hinson, Ella R.;
Cosimi, Lisa;
Borowsky, Mark L.;
Onderdonk, Andrew B.;
Stanley, Sarah A.;
Kaur, Devinder;
Bryant, Kevin F.;
Knipe, David M.;
Sloutsky, Alexander;
Hung, Deborah T.
RNA signatures allow rapid identification of pathogens and antibiotic susceptibilities
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Media type:
E-Article
Title:
RNA signatures allow rapid identification of pathogens and antibiotic susceptibilities
Contributor:
Barczak, Amy K.;
Gomez, James E.;
Kaufmann, Benjamin B.;
Hinson, Ella R.;
Cosimi, Lisa;
Borowsky, Mark L.;
Onderdonk, Andrew B.;
Stanley, Sarah A.;
Kaur, Devinder;
Bryant, Kevin F.;
Knipe, David M.;
Sloutsky, Alexander;
Hung, Deborah T.
Published:
Proceedings of the National Academy of Sciences, 2012
Published in:
Proceedings of the National Academy of Sciences, 109 (2012) 16, Seite 6217-6222
Language:
English
DOI:
10.1073/pnas.1119540109
ISSN:
0027-8424;
1091-6490
Origination:
Footnote:
Description:
With rising rates of drug-resistant infections, there is a need for diagnostic methods that rapidly can detect the presence of pathogens and reveal their susceptibility to antibiotics. Here we propose an approach to diagnosing the presence and drug-susceptibility of infectious diseases based on direct detection of RNA from clinical samples. We demonstrate that species-specific RNA signatures can be used to identify a broad spectrum of infectious agents, including bacteria, viruses, yeast, and parasites. Moreover, we show that the behavior of a small set of bacterial transcripts after a brief antibiotic pulse can rapidly differentiate drug-susceptible and -resistant organisms and that these measurements can be made directly from clinical materials. Thus, transcriptional signatures could form the basis of a uniform diagnostic platform applicable across a broad range of infectious agents.