Spatial separation of Xist RNA and polycomb proteins revealed by superresolution microscopy

Media type: E-Article
Title: Spatial separation of Xist RNA and polycomb proteins revealed by superresolution microscopy
Contributor: Cerase, Andrea; Smeets, Daniel; Tang, Y. Amy; Gdula, Michal; Kraus, Felix; Spivakov, Mikhail; Moindrot, Benoit; Leleu, Marion; Tattermusch, Anna; Demmerle, Justin; Nesterova, Tatyana B.; Green, Catherine; Otte, Arie P.; Schermelleh, Lothar; Brockdorff, Neil
Published: Proceedings of the National Academy of Sciences, 2014
Published in: Proceedings of the National Academy of Sciences, 111 (2014) 6, Seite 2235-2240
Language: English
DOI: 10.1073/pnas.1312951111
ISSN: 0027-8424; 1091-6490
Origination:
Footnote:
Description: Significance Polycomb repressor proteins are recruited to the inactive X chromosome in mammals, and this has been attributed to a biochemical interaction between the non–protein-coding RNA X-inactive specific transcript (Xist), which initiates the X inactivation process, and core polycomb subunits. We have studied this using a combination of genome mapping analysis and 3D structured illumination microscopy (3D-SIM) that allows 3D imaging with eightfold volumetric resolution improvement compared with previous state-of-the-art confocal microscopy. Our findings reveal that Xist-mediated recruitment of polycomb repressors does not correlate well with gene silencing and, moreover, that using 3D-SIM, polycomb proteins and Xist RNA show significant spatial separation. These observations challenge prevailing models and prompt a reappraisal of the role of Xist RNA in polycomb recruitment.
Access State: Open Access

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