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Media type:
E-Article
Title:
A distinct RNA recognition mechanism governs Np 4 decapping by RppH
Contributor:
Levenson-Palmer, Rose;
Luciano, Daniel J.;
Vasilyev, Nikita;
Nuthanakanti, Ashok;
Serganov, Alexander;
Belasco, Joel G.
Published:
Proceedings of the National Academy of Sciences, 2022
Published in:
Proceedings of the National Academy of Sciences, 119 (2022) 6
Language:
English
DOI:
10.1073/pnas.2117318119
ISSN:
0027-8424;
1091-6490
Origination:
Footnote:
Description:
Significance Dinucleoside tetraphosphate alarmones function in bacteria as precursors to 5′-terminal nucleoside tetraphosphate (Np 4 ) caps, becoming incorporated at high levels into RNA during stress and thereby influencing transcript lifetimes. However, little is known about how these noncanonical caps are removed as a prelude to RNA degradation. Here, we report that the RNA pyrophosphohydrolase RppH assumes a leading role in decapping those transcripts under conditions of disulfide stress and that it recognizes Np 4 -capped 5′ ends by an unexpected mechanism, generating a triphosphorylated RNA intermediate that must undergo further deprotection by RppH to trigger degradation. These findings help to explain the uneven distribution of Np 4 caps on bacterial transcripts and have important implications for how gene expression is reprogrammed in response to stress.