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Media type:
E-Article
Title:
Submillimolar levels of calcium regulates DNA structure at the dinucleotide repeat (TG/AC) n
Contributor:
Dobi, A.;
v. Agoston, D.
imprint:
Proceedings of the National Academy of Sciences, 1998
Published in:Proceedings of the National Academy of Sciences
Language:
English
DOI:
10.1073/pnas.95.11.5981
ISSN:
0027-8424;
1091-6490
Origination:
Footnote:
Description:
<jats:p>
Submillimolar levels of calcium, similar to the physiological total (bound + free) intranuclear concentration (0.01–1 mM), induced a conformational change within d(TG/AC)
<jats:sub>n</jats:sub>
, one of the frequent dinucleotide repeats of the mammalian genome. This change is calcium-specific, because no other tested cation induced it and it was detected as a concentration-dependent transition from B- to a non-B-DNA conformation expanding from 3′ end toward the 5′ of the repeat. Genomic footprinting of various rat brain regions revealed the existence of similar non-B-DNA conformation within a d(TG/AC)
<jats:sub>28</jats:sub>
repeat of the endogenous enkephalin gene only in enkephalin-expressing caudate nucleus and not in the nonexpressing thalamus. Binding assays demonstrated that DNA could bind calcium and can compete with calmodulin for calcium.
</jats:p>