Published in:
Bioscience, Biotechnology, and Biochemistry, 78 (2014) 8, Seite 1384-1388
Language:
English
DOI:
10.1080/09168451.2014.918490
ISSN:
0916-8451;
1347-6947
Origination:
Footnote:
Description:
Abstract We evaluated the substrate specificities of four proline cis-selective hydroxylases toward the efficient synthesis of proline derivatives. In an initial evaluation, 15 proline-related compounds were investigated as substrates. In addition to l-proline and l-pipecolinic acid, we found that 3,4-dehydro-l-proline, l-azetidine-2-carboxylic acid, cis-3-hydroxy-l-proline, and l-thioproline were also oxygenated. Subsequently, the product structures were determined, revealing cis-3,4-epoxy-l-proline, cis-3-hydroxy-l-azetidine-2-carboxylic acid, and 2,3-cis-3,4-cis-3,4-dihydroxy-l-proline.