Parisi, Alessio;
Struelens, Lara;
Vanhavere, Filip
Comparison between the results of a recently-developed biological weighting function (V79-RBE10 BWF) and the in vitro clonogenic survival RBE10 of other repair-competent asynchronized normoxic mammalian cell lines and ions not used for the development of the model
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Media type:
E-Article
Title:
Comparison between the results of a recently-developed biological weighting function (V79-RBE10 BWF) and the in vitro clonogenic survival RBE10 of other repair-competent asynchronized normoxic mammalian cell lines and ions not used for the development of the model
Contributor:
Parisi, Alessio;
Struelens, Lara;
Vanhavere, Filip
Published:
IOP Publishing, 2021
Published in:
Physics in Medicine & Biology, 66 (2021) 23, Seite 235006
Description:
<jats:title>Abstract</jats:title>
<jats:p>728 simulated microdosimetric lineal energy spectra (26 different ions between <jats:sup>1</jats:sup>H and <jats:sup>238</jats:sup>U, 28 energy points from 1 to 1000 MeV/n) were used in combination with a recently-developed biological weighting function (Parisi <jats:italic>et al</jats:italic> 2020 <jats:italic>Phys. Med. Biol.</jats:italic> 1361–6560) and 571 published <jats:italic>in vitro</jats:italic> clonogenic survival curves in order to: (1) assess prediction intervals for the <jats:italic>in silico</jats:italic> results by deriving an empirical indication of the experimental uncertainty from the dispersion in the <jats:italic>in vitro</jats:italic> hamster lung fibroblast (V79) data used for the development of the biophysical model; (2) explore the possibility of modeling the relative biological effectiveness (RBE) of the 10% clonogenic survival of asynchronized normoxic repair-competent mammalian cell lines other than the one used for the development of the model (V79); (3) investigate the predictive power of the model through a comparison between <jats:italic>in silico</jats:italic> results and <jats:italic>in vitro</jats:italic> data for 10 ions not used for the development of the model. At first, different strategies for the assessment of the <jats:italic>in silico</jats:italic> prediction intervals were compared. The possible sources of uncertainty responsible for the dispersion in the <jats:italic>in vitro</jats:italic> data were also shortly reviewed. Secondly, also because of the relevant scatter in the <jats:italic>in vitro</jats:italic> data, no statistically-relevant differences were found between the RBE<jats:sub>10</jats:sub> of the investigated different asynchronized normoxic repair-competent mammalian cell lines. The only exception (Chinese Hamster peritoneal fibroblasts, B14FAF28), is likely due to the limited dataset (all <jats:italic>in vitro</jats:italic> ion data were extracted from a single publication), systematic differences in the linear energy transfer calculations for the employed very-heavy ions, and the use of reference photon survival curves extracted from a different publication. Finally, the <jats:italic>in silico</jats:italic> predictions for the 10 ions not used for the model development were in good agreement with the corresponding <jats:italic>in vitro</jats:italic> data.</jats:p>