Knudsen, Maja Johanne Søndergaard;
Samaniego Castruita, Jose Alfredo;
Mollerup, Sarah;
Holzknecht, Barbara Juliane;
Hoppe, Morten;
Westh, Henrik;
Pinholt, Mette;
Rubin, Ingrid Maria Cecilia
Long-term carriage and evolution of vancomycin-resistant Enterococcus faecium: a genomic study on consecutive isolates
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Media type:
E-Article
Title:
Long-term carriage and evolution of vancomycin-resistant Enterococcus faecium: a genomic study on consecutive isolates
Contributor:
Knudsen, Maja Johanne Søndergaard;
Samaniego Castruita, Jose Alfredo;
Mollerup, Sarah;
Holzknecht, Barbara Juliane;
Hoppe, Morten;
Westh, Henrik;
Pinholt, Mette;
Rubin, Ingrid Maria Cecilia
Description:
<jats:title>Abstract</jats:title>
<jats:sec>
<jats:title>Objectives</jats:title>
<jats:p>To determine if vancomycin-resistant Enterococcus faecium (VREfm) carriers carry the same VREfm clone after a minimum follow-up of 365 days. For those carrying the same clone, we investigated the genomic evolution per year per genome.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Methods</jats:title>
<jats:p>We used WGS results to assign VREfm clones to each isolate and determine clone shifts. Finally, we calculated distance in core-genome MLST alleles, and the number of SNPs between consecutive VREfm isolates from patients carrying the same VREfm clone.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Results</jats:title>
<jats:p>In total, 44.2% of patients carried the same VREfm clone, and the genomic evolution was 1.8 alleles and 2.6 SNPs per genome per year.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Conclusions</jats:title>
<jats:p>In our population of long-term carriers, we calculated a molecular clock of 2.6 SNPs.</jats:p>
</jats:sec>