Description:
<jats:p>Allogenic whole cancer cell vaccines have been proposed for cancer immunotherapy. Immunogenic cell death (ICD) is one of programed cell death and the ICD induced cells show high immunogenicity and can activate immune system effectively. So, ICD induced cancer cell is expected as an autologous cell vaccine. As ICD induction method, chemical and physical methods have been proposed. Among them, high hydrostatic pressure (HHP) treatment can induce ICD of cancer cell. The HHP‐ICD induced cancer cells show higher immune activation than other methods. However, the ICD induction efficiency is still low. Therefore, in order to develop simple and effective ICD induction method, we investigated ICD induction method by using various detergents, such as SDS, Triton X‐100 and Tween 20. HeLa cells were treated with detergents at various concentrations. For the Tween 20 treated HeLa cell, the calreticulin and HMGB1 translocations were confirmed by confocal laser microscopic observation (Fig. 1). This result indicates that immunogenic cell death of HeLa cells was induced by the detergent treatment. For SDS and Triton X‐100, although the cell death was induced, the calreticulin and HMGB1 translocations were not confirmed. The ICD induced HeLa cells were effectively phagocytosed by macrophage‐like cells and the effective secretion of TNF‐alpha from the macrophage‐like cells also confirmed by ELIZA assay (Fig. 2). From these results, it was suggested that the ICD inducted cancer cells by using Tween 20 could activate immune system.</jats:p><jats:p><jats:bold>Support or Funding Information</jats:bold></jats:p><jats:p>JSPS Grant‐in‐Aid for Scientific Research (B)</jats:p><jats:p>This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in <jats:italic>The FASEB Journal</jats:italic>.</jats:p>