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Media type:
E-Article
Title:
At the molecular resolution with MINFLUX?
Contributor:
Prakash, Kirti
imprint:
The Royal Society, 2022
Published in:Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences
Language:
English
DOI:
10.1098/rsta.2020.0145
ISSN:
1364-503X;
1471-2962
Origination:
Footnote:
Description:
<jats:p>MINFLUX is purported as the next revolutionary fluorescence microscopy technique claiming a spatial resolution in the range of 1–3 nm in fixed and living cells. Though the claim of molecular resolution is attractive, I am concerned whether true 1 nm resolution has been attained. Here, I compare the performance with other super-resolution methods focusing particularly on spatial resolution claims, subjective filtering of localizations, detection versus labelling efficiency and the possible limitations when imaging biological samples containing densely labelled structures. I hope the analysis and evaluation parameters presented here are not only useful for future research directions for single-molecule techniques but also microscope users, developers and core facility managers when deciding on an investment for the next ‘state-of-the-art’ instrument.</jats:p>
<jats:p>This article is part of the Theo Murphy meeting issue ‘Super-resolution structured illumination microscopy (part 2)’.</jats:p>