Veldhuis Kroeze, Edwin J. B.;
van Amerongen, Geert;
Dijkshoorn, Marcel L.;
Simon, James H.;
de Waal, Leon;
Hartmann, Ieneke J. C.;
Krestin, Gabriel P.;
Kuiken, Thijs;
Osterhaus, Albert D. M. E.;
Stittelaar, Koert J.
Pulmonary pathology of pandemic influenza A/H1N1 virus (2009)-infected ferrets upon longitudinal evaluation by computed tomography
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Media type:
E-Article
Title:
Pulmonary pathology of pandemic influenza A/H1N1 virus (2009)-infected ferrets upon longitudinal evaluation by computed tomography
Contributor:
Veldhuis Kroeze, Edwin J. B.;
van Amerongen, Geert;
Dijkshoorn, Marcel L.;
Simon, James H.;
de Waal, Leon;
Hartmann, Ieneke J. C.;
Krestin, Gabriel P.;
Kuiken, Thijs;
Osterhaus, Albert D. M. E.;
Stittelaar, Koert J.
Published:
Microbiology Society, 2011
Published in:
Journal of General Virology, 92 (2011) 8, Seite 1854-1858
Language:
English
DOI:
10.1099/vir.0.032805-0
ISSN:
0022-1317;
1465-2099
Origination:
Footnote:
Description:
We investigated the development of pulmonary lesions in ferrets by means of computed tomography (CT) following infection with the 2009 pandemic A/H1N1 influenza virus and compared the scans with gross pathology, histopathology and immunohistochemistry. Ground-glass opacities observed by CT scanning in all infected lungs corresponded to areas of alveolar oedema at necropsy. These areas were most pronounced on day 3 and gradually decreased from days 4 to 7 post-infection. This pilot study shows that the non-invasive imaging procedure allows quantification and characterization of influenza-induced pulmonary lesions in living animals under biosafety level 3 conditions and can thus be used in pre-clinical pharmaceutical efficacy studies.