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Decano, Arun Gonzales; Tran, Nghia; Al-Foori, Hawriya; Al-Awadi, Buthaina; Campbell, Leigh; Ellison, Kevin; Mirabueno, Louisse Paolo; Nelson, Maddy; Power, Shane; Smith, Genevieve; Smyth, Cian; Vance, Zoe; Woods, Caitriona; Rahm, Alexander; Downing, Tim

Plasmids shape the diverse accessory resistomes of Escherichia coli ST131

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  • Media type: E-Article
  • Title: Plasmids shape the diverse accessory resistomes of Escherichia coli ST131
  • Contributor: Decano, Arun Gonzales; Tran, Nghia; Al-Foori, Hawriya; Al-Awadi, Buthaina; Campbell, Leigh; Ellison, Kevin; Mirabueno, Louisse Paolo; Nelson, Maddy; Power, Shane; Smith, Genevieve; Smyth, Cian; Vance, Zoe; Woods, Caitriona; Rahm, Alexander; Downing, Tim
  • imprint: Microbiology Society, 2021
  • Published in: Access Microbiology
  • Language: English
  • DOI: 10.1099/acmi.0.000179
  • ISSN: 2516-8290
  • Origination:
  • Footnote:
  • Description: <jats:p>The human gut microbiome includes beneficial, commensal and pathogenic bacteria that possess antimicrobial resistance (AMR) genes and exchange these predominantly through conjugative plasmids. <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.3093" xlink:type="simple">Escherichia coli</jats:ext-link> </jats:named-content> </jats:italic> is a significant component of the gastrointestinal microbiome and is typically non-pathogenic in this niche. In contrast, extra-intestinal pathogenic <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.3093" xlink:type="simple">E. coli</jats:ext-link> </jats:named-content> </jats:italic> (ExPEC) including ST131 may occupy other environments like the urinary tract or bloodstream where they express genes enabling AMR and host cell adhesion like type 1 fimbriae. The extent to which commensal <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.3093" xlink:type="simple">E. coli</jats:ext-link> </jats:named-content> </jats:italic> and uropathogenic ExPEC ST131 share AMR genes remains understudied at a genomic level, and we examined this here using a preterm infant resistome. We found that individual ST131 had small differences in AMR gene content relative to a larger shared resistome. Comparisons with a range of plasmids common in ST131 showed that AMR gene composition was driven by conjugation, recombination and mobile genetic elements. Plasmid pEK499 had extended regions in most ST131 Clade C isolates, and it had evidence of a co-evolutionary signal based on protein-level interactions with chromosomal gene products, as did pEK204 that had a type IV fimbrial <jats:italic>pil</jats:italic> operon. ST131 possessed extensive diversity of selective type 1, type IV, P and F17-like fimbriae genes that was highest in subclade C2. The structure and composition of AMR genes, plasmids and fimbriae vary widely in ST131 Clade C and this may mediate pathogenicity and infection outcomes.</jats:p>
  • Access State: Open Access