Arthrobacter bussei sp. nov., a pink-coloured organism isolated from cheese made of cow’s milk

Media type: E-Article
Title: Arthrobacter bussei sp. nov., a pink-coloured organism isolated from cheese made of cow’s milk
Contributor: Flegler, Alexander; Runzheimer, Katharina; Kombeitz, Vanessa; Mänz, Anna Tatjana; Heidler von Heilborn, David; Etzbach, Lara; Schieber, Andreas; Hölzl, Georg; Hüttel, Bruno; Woehle, Christian; Lipski, André
imprint: Microbiology Society, 2020
Published in: International Journal of Systematic and Evolutionary Microbiology
Language: English
DOI: 10.1099/ijsem.0.004125
ISSN: 1466-5026; 1466-5034
Keywords: General Medicine ; Ecology, Evolution, Behavior and Systematics ; Microbiology
Origination:
Footnote:
Description: <jats:p>A pink-coloured bacterium (strain KR32<jats:sup>T</jats:sup>) was isolated from cheese and assigned to the ‘<jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.5841" xlink:type="simple">Arthrobacter agilis</jats:ext-link> </jats:named-content> </jats:italic> group’. Members of the ‘pink <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.5841" xlink:type="simple">Arthrobacter agilis</jats:ext-link> </jats:named-content> </jats:italic> group’ form a stable clade (100 % bootstrap value) and contain the species <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.5841" xlink:type="simple">Arthrobacter agilis</jats:ext-link> </jats:named-content> </jats:italic>, <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.31683" xlink:type="simple">Arthrobacter ruber</jats:ext-link> </jats:named-content> </jats:italic> and <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.28603" xlink:type="simple">Arthrobacter echini</jats:ext-link> </jats:named-content> </jats:italic>, which share ≥99.0 % 16S rRNA gene sequence similarity. Isolate KR32<jats:sup>T</jats:sup> showed highest 16S rRNA gene sequence similarity (99.9 %) to <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.5841" xlink:type="simple">A. agilis</jats:ext-link> </jats:named-content> </jats:italic> DSM 20550<jats:sup>T</jats:sup>. Additional multilocus sequence comparison confirmed the assignment of strain KR32<jats:sup>T</jats:sup> to the clade ‘pink <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.5841" xlink:type="simple">A. agilis</jats:ext-link> </jats:named-content> </jats:italic> group’. Average nucleotide identity and digital DNA–DNA hybridization values between isolate KR32<jats:sup>T</jats:sup> and <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.5841" xlink:type="simple">A. agilis</jats:ext-link> </jats:named-content> </jats:italic> DSM 20550<jats:sup>T</jats:sup> were 82.85 and 26.30 %, respectively. The G+C content of the genomic DNA of isolate KR32<jats:sup>T</jats:sup> was 69.14 mol%. Chemotaxonomic analysis determined anteiso-C<jats:sub>15 : 0</jats:sub> as the predominant fatty acid and MK-9(H<jats:sub>2</jats:sub>) as the predominant menaquinone. Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and monoacyldimannosyl-monoacylglycerol. The peptidoglycan type of the isolate was A3α. The carotenoid bacterioruberin was detected as the major pigment. At 10 °C, strain KR32<jats:sup>T</jats:sup> grew with increased concentrations of bacterioruberin and production of unsaturated fatty acids. Strain KR32<jats:sup>T</jats:sup> was a Gram-stain-positive, catalase-positive, oxidase-positive and coccus-shaped bacterium with optimal growth at 27–30 °C and pH 8. The results of phylogenetic and phenotypic analyses enabled the differentiation of the isolate from other closely related species of the ‘pink <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.5841" xlink:type="simple">A. agilis</jats:ext-link> </jats:named-content> </jats:italic> group’. Therefore, strain KR32<jats:sup>T</jats:sup> represents a novel species for which the name <jats:italic>Arthrobacter bussei</jats:italic> sp. nov. is proposed. The type strain is KR32<jats:sup>T</jats:sup> (=DSM 109896<jats:sup>T</jats:sup>=LMG 31480<jats:sup>T</jats:sup>=NCCB 100733<jats:sup>T</jats:sup>).</jats:p>
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