Description:
<jats:p>A novel Gram-negative, obligate aerobic, mobile, rod-shaped and non-spore-forming bacterial strain, WCHP16<jats:sup>T</jats:sup>, was isolated from the wastewater treatment plant at West China Hospital in Chengdu, PR China. It was characterized using a polyphasic approach. Analysis of its 16S rRNA gene sequence showed that strain WCHP16<jats:sup>T</jats:sup> belonged to the genus <jats:italic>
<jats:named-content content-type="genus">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.2552" xlink:type="simple">Pseudomonas</jats:ext-link>
</jats:named-content>
</jats:italic> with the highest similarity to <jats:italic>Pseudomonas qingdaonensis</jats:italic> JJ3<jats:sup>T</jats:sup> (99.34 %), <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.35540" xlink:type="simple">Pseudomonas shirazica</jats:ext-link>
</jats:named-content>
</jats:italic> VM14<jats:sup>T</jats:sup> (99.0 %), <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.2666" xlink:type="simple">Pseudomonas plecoglossicida</jats:ext-link>
</jats:named-content>
</jats:italic> NBRC 103162<jats:sup>T</jats:sup> (99.0 %) and <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.34407" xlink:type="simple">Pseudomonas asiatica</jats:ext-link>
</jats:named-content>
</jats:italic> RYU5<jats:sup>T</jats:sup> (99.0 %). Phylogenomic analysis based on 107 core gene sequences demonstrated that WCHP16<jats:sup>T</jats:sup> was a member of the <jats:italic>
<jats:named-content content-type="species">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.2674" xlink:type="simple">Pseudomonas putida</jats:ext-link>
</jats:named-content>
</jats:italic> group but was distant from all closely related species. Whole-genome comparisons, using average nucleotide identity based on <jats:sc>blast</jats:sc> (ANIb) and <jats:italic>in silico</jats:italic> DNA–DNA hybridization (<jats:italic>is</jats:italic>DDH), confirmed low genome relatedness to all the known <jats:italic>
<jats:named-content content-type="genus">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.2552" xlink:type="simple">Pseudomonas</jats:ext-link>
</jats:named-content>
</jats:italic> species (below the recommended thresholds of 95 % [ANIb] and 70 % [<jats:italic>is</jats:italic>DDH] for species delineation). Phenotypic characterization tests showed that the utilization of phenylacetic acid and capric acid, but not <jats:sc>d</jats:sc>-arabitol, and inability to produce fluorescent (King B medium) in combination could distinguish this strain from other related species of the genus <jats:italic>
<jats:named-content content-type="genus">
<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.2552" xlink:type="simple">Pseudomonas</jats:ext-link>
</jats:named-content>
</jats:italic>. Therefore, based on genetic and phenotypic evidence, it is clear that strain WCHP16<jats:sup>T</jats:sup> represents a novel species, for which the name <jats:italic>Pseudomonas defluvii</jats:italic> sp. nov. is proposed. The type strain is WCHP16<jats:sup>T</jats:sup> (GDMCC1.1215<jats:sup>T</jats:sup>=CCTCC AB 2017103<jats:sup>T</jats:sup>=KCTC 52991<jats:sup>T</jats:sup>).</jats:p>