Description:
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<jats:bold>Introduction.</jats:bold> Identification of non-O157 Shiga-toxin-producing <jats:italic>Escherichia coli</jats:italic> (STEC) infections may be underestimated in microbiological diagnosis.</jats:p>
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<jats:bold>Case presentation.</jats:bold> A 58-year-old woman developed diarrhoea with watery and subsequently mucous stool. Initial multiplex PCR testing revealed a positive result for <jats:italic>stx<jats:sub>2</jats:sub>
</jats:italic>. Culture isolation of a STEC was successful only after repeated inoculation of chromogenic <jats:italic>E. coli</jats:italic> media. Molecular characterization was performed and identified the isolate as <jats:italic>stx</jats:italic>
<jats:sub>2e</jats:sub>-positive STEC of serotype O8 : H19. The strain harboured <jats:italic>lpfA</jats:italic>, but not <jats:italic>eae</jats:italic>.</jats:p>
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<jats:bold>Conclusion.</jats:bold> This case highlights the usefulness of initial multiplex PCR for diagnosis of non-O157 STEC infection.</jats:p>