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Bossé, Janine T.; Li, Yanwen; Cohen, Liza Miriam; Stegger, Marc; Angen, Øystein; Lacouture, Sonia; Gottschalk, Marcelo; Lei, Liancheng; Koene, Miriam; Kuhnert, Peter; Bandara, Aloka B.; Inzana, Thomas J.; Holden, Matthew T. G.; Harris, David; Oshota, Olusegun; Maskell, Duncan J.; Tucker, Alexander W.; Wren, Brendan W.; Rycroft, Andrew N.; Langford, Paul R.

Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation

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  • Media type: E-Article
  • Title: Complete genome for Actinobacillus pleuropneumoniae serovar 8 reference strain 405: comparative analysis with draft genomes for different laboratory stock cultures indicates little genetic variation
  • Contributor: Bossé, Janine T.; Li, Yanwen; Cohen, Liza Miriam; Stegger, Marc; Angen, Øystein; Lacouture, Sonia; Gottschalk, Marcelo; Lei, Liancheng; Koene, Miriam; Kuhnert, Peter; Bandara, Aloka B.; Inzana, Thomas J.; Holden, Matthew T. G.; Harris, David; Oshota, Olusegun; Maskell, Duncan J.; Tucker, Alexander W.; Wren, Brendan W.; Rycroft, Andrew N.; Langford, Paul R.
  • imprint: Microbiology Society, 2021
  • Published in: Microbial Genomics
  • Language: English
  • DOI: 10.1099/mgen.0.000687
  • ISSN: 2057-5858
  • Keywords: General Medicine
  • Origination:
  • Footnote:
  • Description: <jats:p>We report here the complete genome sequence of the widely studied <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.3408" xlink:type="simple">Actinobacillus pleuropneumoniae</jats:ext-link> </jats:named-content> </jats:italic> serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, including the same original stock used to generate the PacBio sequence, held in different countries and found little genetic variation, with only three SNPs identified, all within the <jats:italic>degS</jats:italic> gene. However, sequences of two small plasmids, pARD3079 and p405tetH, detected by Illumina sequencing of the draft genomes were not identified in the PacBio sequence of the reference strain.</jats:p>
  • Access State: Open Access