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Media type:
E-Article
Title:
A polyketide synthase gene required for ochratoxin A biosynthesis in Aspergillus ochraceus
Contributor:
O'Callaghan, J.;
Caddick, M. X.;
Dobson, A. D. W.
imprint:
Microbiology Society, 2003
Published in:Microbiology
Language:
English
DOI:
10.1099/mic.0.26619-0
ISSN:
1350-0872;
1465-2080
Origination:
Footnote:
Description:
<jats:p>Ochratoxin A is an important nephrotoxic and nephrocarcinogenic mycotoxin, produced by <jats:italic>Aspergillus ochraceus</jats:italic> as a polyketide-derived secondary metabolite. A portion of a putative polyketide synthase gene (<jats:italic>pks</jats:italic>) involved in the biosynthesis of this mycotoxin was cloned by using a suppression subtractive hybridization PCR-based approach. The predicted amino acid sequence of the 1·4 kb clone shared 28–35 % identity to acyl transferase regions from fungal polyketide synthases found in the databases. Based on reverse transcription PCR studies, the <jats:italic>pks</jats:italic> gene is expressed only under ochratoxin A permissive conditions and only during the early stages of the mycotoxin synthesis. A mutant in which the <jats:italic>pks</jats:italic> gene has been interrupted cannot synthesize ochratoxin A. This report is the first of the cloning and characterization of a gene involved in ochratoxin A biosynthesis.</jats:p>