The impact of different intensities of green light on the bacteriochlorophyll homologue composition of the chlorobiaceae Prosthecochloris aestuarii and Chlorobium phaeobacteroides
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Media type:
E-Article
Title:
The impact of different intensities of green light on the bacteriochlorophyll homologue composition of the chlorobiaceae Prosthecochloris aestuarii and Chlorobium phaeobacteroides
Description:
Members of theChlorobiaceaeandChloroflexaceaeare unique among the phototrophic micro-organisms in having a remarkably rich chlorophyll pigment diversity. The physiological regulation of this diversity and its ecological implications are still enigmatic. The bacteriochlorophyll composition of the chlorobiaceaeProsthecochloris aestuariistrain CE 2404 andChlorobium phaeobacteroidesstrain UdG 6030 was therefore studied by both HPLC with photodiode array (PDA) detection and liquid chromatography-mass spectrometry (LC-MS). These strains were grown in liquid cultures under green light (480–615 nm) at different light intensities (0·2–55·7 μmol photons m−2 s−1), simulating the irradiance regime at different depths of the water column of deep lakes. The specific growth rates ofPtc. aestuariiunder green light achieved a maximum of 0·06 h−1at light intensities exceeding 6 μmol photons m−2 s−1, lower than the maximum observed under white light (approx. 0·1 h−1). The maximal growth rates ofChl. phaeobacteroidesunder green light were slightly higher (0·07 h−1) than observed forPtc. aestuariiand were achieved at 3·5 and 4·3 μmol photons m−2 s−1. LC-MS/MS analysis of pigment extracts revealed most (>90 %) BChlchomologues ofPtc. aestuariito be esterified with farnesol. The homologues differed in mass by multiples of 14 Da, reflecting different alkyl subsituents at positions C-8 and C-12 on the tetrapyrrole macrocycle. The relative proportions of the individual homologues varied only slightly among different light intensities. The specific content of BChlcwas maximal at 3–5 μmol photons m−2 s−1[400±150 nmol BChlc(mg protein)−1]. In the case ofChl. phaeobacteroides, the specific content of BChlewas maximal at 4·3 μmol photons m−2 s−1[115 nmol BChle(mg protein)−1], and this species was characterized by high carotenoid (isorenieratene) contents. The major BChleforms were esterified with a range of isoprenoid and straight-chain alcohols. The major isoprenoid alcohols comprised mainly farnesol and to a lesser extent geranylgeraniol. The straight-chain alcohols included C15, C15 : 1, C16, C16 : 1and C17. Interestingly, the proportion of straight alkyl chains over isoprenoid esterified side chains shifted markedly with increasing light intensity: the isoprenoid side chains dominated at low light intensities, while the straight-chain alkyl substituents dominated at higher light intensities. The authors propose that this phenomenon may be explained as a result of changing availability of reducing power, i.e. the highly reduced straight-chain alcohols have a higher biosynthetic demand for NADPH2than the polyunsaturated isoprenoid with the same number of carbon atoms.