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Davydenko, Svetlana G.; Feng, DeJiang; Jäntti, Jussi; Keränen, Sirkka

Characterization of GPI14/YJR013w mutation that induces the cell wall integrity signalling pathway and results in increased protein production in Saccharomyces cerevisiae

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  • Media type: E-Article
  • Title: Characterization of GPI14/YJR013w mutation that induces the cell wall integrity signalling pathway and results in increased protein production in Saccharomyces cerevisiae
  • Contributor: Davydenko, Svetlana G.; Feng, DeJiang; Jäntti, Jussi; Keränen, Sirkka
  • imprint: Wiley, 2005
  • Published in: Yeast
  • Language: English
  • DOI: 10.1002/yea.1286
  • ISSN: 0749-503X; 1097-0061
  • Keywords: Genetics ; Applied Microbiology and Biotechnology ; Biochemistry ; Bioengineering ; Biotechnology
  • Origination:
  • Footnote:
  • Description: <jats:title>Abstract</jats:title><jats:p>We report here identification and characterization of a mutation in the <jats:italic>GPI14</jats:italic> gene, the yeast homologue of the mammalian PIG‐M that functions in the synthesis of the GPI moiety anchoring proteins to the plasma membrane. We show that the first putative transmembrane domain of Gpi14p is not essential for its function. Downregulation of <jats:italic>GPI14</jats:italic> expression/reduced protein function due to an amino terminal deletion resulted in increased transcription and production of an endogenous and a heterologous secreted protein expressed from <jats:italic>HSP150</jats:italic> and <jats:italic>ADH1</jats:italic> promoter, respectively. In these cells, unfolded protein response was induced but was not responsible for the enhanced production of these proteins. A cell wall defect in the <jats:italic>gpi14</jats:italic> mutant cells was suggested by cell aggregation phenotype, increased sensitivity to Calcofluor white, an increased release of Gas1p and total protein into the culture medium. In the <jats:italic>gpi14</jats:italic> mutant cells, transcription of <jats:italic>RLM1</jats:italic>, a transcription factor participating in the cell wall integrity signalling pathway, was increased, and deletion of <jats:italic>RLM1</jats:italic> resulted in a synthetic lethal phenotype with the <jats:italic>gpi14</jats:italic> mutation. These results suggest that partial inactivation of Gpi14p causes defects in the cell wall structure and suggest that compromised GPI anchor synthesis results in enhanced protein production via the cell wall integrity signalling pathway. Copyright © 2005 John Wiley &amp; Sons, Ltd.</jats:p>
  • Access State: Open Access