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Media type:
E-Article
Title:
Targeted siRNA Delivery Using a Lipo‐Oligoaminoamide Nanocore with an Influenza Peptide and Transferrin Shell
Contributor:
Zhang, Wei;
Müller, Katharina;
Kessel, Eva;
Reinhard, Sören;
He, Dongsheng;
Klein, Philipp M.;
Höhn, Miriam;
Rödl, Wolfgang;
Kempter, Susanne;
Wagner, Ernst
Published:
Wiley, 2016
Published in:
Advanced Healthcare Materials, 5 (2016) 12, Seite 1493-1504
Language:
English
DOI:
10.1002/adhm.201600057
ISSN:
2192-2640;
2192-2659
Origination:
Footnote:
Description:
Developing RNA‐interference‐based therapeutic approaches with efficient and targeted cytosolic delivery of small interfering RNA (siRNA) is remaining a critical challenge since two decades. Herein, a multifunctional transferrin receptor (TfR)‐targeted siRNA delivery system (Tf&INF7) is designed based on siRNA complexes formed with the cationic lipo‐oligoamino amide 454, sequentially surface‐modified with polyethylene glycol‐linked transferrin (Tf) for receptor targeting and the endosomolytic peptide INF7 for efficient cytosolic release of the siRNA. Effective Tf&INF7 polyplex internalization and target gene silencing are demonstrated for the TfR overexpressing tumor cell lines (K562, D145, and N2a). Treatment with antitumoral EG5 siRNA results in a block of tumor cell growth and triggered apoptosis. Tf‐modified polyplexes are far more effective than the corresponding albumin‐ (Alb) or nonmodified 454 polyplexes. Competition experiments with excess of Tf demonstrate TfR target specificity. As alternative to the ligand Tf, an anti‐murine TfR antibody is incorporated into the polyplexes for specific targeting and gene silencing in the murine N2a cell line. In vivo distribution studies not only demonstrate an enhanced tumor residence of siRNA in N2a tumor‐bearing mice with the Tf&INF7 as compared to the 454 polyplex group but also a reduced siRNA nanoparticle stability limiting the in vivo performance.