Schmidt, Samuel;
Adjobo‐Hermans, Merel J. W.;
Wallbrecher, Rike;
Verdurmen, Wouter P. R.;
Bovée‐Geurts, Petra H. M.;
van Oostrum, Jenny;
Milletti, Francesca;
Enderle, Thilo;
Brock, Roland
Detecting Cytosolic Peptide Delivery with the GFP Complementation Assay in the Low Micromolar Range
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Media type:
E-Article
Title:
Detecting Cytosolic Peptide Delivery with the GFP Complementation Assay in the Low Micromolar Range
Contributor:
Schmidt, Samuel;
Adjobo‐Hermans, Merel J. W.;
Wallbrecher, Rike;
Verdurmen, Wouter P. R.;
Bovée‐Geurts, Petra H. M.;
van Oostrum, Jenny;
Milletti, Francesca;
Enderle, Thilo;
Brock, Roland
Published:
Wiley, 2015
Published in:
Angewandte Chemie, 127 (2015) 50, Seite 15320-15323
Language:
German
DOI:
10.1002/ange.201505913
ISSN:
0044-8249;
1521-3757
Origination:
Footnote:
Description:
AbstractTransfection of cells with a plasmid encoding for the first ten strands of the GFP protein (GFP1‐10) provides the means to detect cytosolic peptide import at low micromolar concentrations. Cytosolic import of the eleventh strand of the GFP protein either by electroporation or by cell‐penetrating peptide‐mediated import leads to formation of the full‐length GFP protein and fluorescence. An increase in sensitivity is achieved through structural modifications of the peptide and the expression of GFP1‐10 as a fusion protein with mCherry.