Nielsen, Thorbjørn B.;
Thomsen, Rasmus P.;
Mortensen, Michael R.;
Kjems, Jørgen;
Nielsen, Per Franklin;
Nielsen, Thomas E.;
Kodal, Anne Louise B.;
Cló, Emiliano;
Gothelf, Kurt V.
Peptide‐Directed DNA‐Templated Protein Labelling for The Assembly of a Pseudo‐IgM
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Media type:
E-Article
Title:
Peptide‐Directed DNA‐Templated Protein Labelling for The Assembly of a Pseudo‐IgM
Contributor:
Nielsen, Thorbjørn B.;
Thomsen, Rasmus P.;
Mortensen, Michael R.;
Kjems, Jørgen;
Nielsen, Per Franklin;
Nielsen, Thomas E.;
Kodal, Anne Louise B.;
Cló, Emiliano;
Gothelf, Kurt V.
imprint:
Wiley, 2019
Published in:
Angewandte Chemie, 131 (2019) 27, Seite 9166-9170
Description:
<jats:title>Abstract</jats:title><jats:p>The development of methods for conjugation of DNA to proteins is of high relevance for the integration of protein function and DNA structures. Here, we demonstrate that protein‐binding peptides can direct a DNA‐templated reaction, selectively furnishing DNA–protein conjugates with one DNA label. Quantitative conversion of oligonucleotides is achieved at low stoichiometries and the reaction can be performed in complex biological matrixes, such as cell lysates. Further, we have used a star‐like pentameric DNA nanostructure to assemble five DNA–Rituximab conjugates, made by our reported method, into a pseudo‐IgM antibody structure that was subsequently characterized by negative‐stain transmission electron microscopy (nsTEM) analysis.</jats:p>