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Joensson, Haakan N.; Samuels, Michael L.; Brouzes, Eric R.; Medkova, Martina; Uhlén, Mathias; Link, Darren R.; Andersson‐Svahn, Helene

Detection and Analysis of Low‐Abundance Cell‐Surface Biomarkers Using Enzymatic Amplification in Microfluidic Droplets

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  • Media type: E-Article
  • Title: Detection and Analysis of Low‐Abundance Cell‐Surface Biomarkers Using Enzymatic Amplification in Microfluidic Droplets
  • Contributor: Joensson, Haakan N.; Samuels, Michael L.; Brouzes, Eric R.; Medkova, Martina; Uhlén, Mathias; Link, Darren R.; Andersson‐Svahn, Helene
  • Published: Wiley, 2009
  • Published in: Angewandte Chemie International Edition, 48 (2009) 14, Seite 2518-2521
  • Language: English
  • DOI: 10.1002/anie.200804326
  • ISSN: 1433-7851; 1521-3773
  • Origination:
  • Footnote:
  • Description: <jats:title>Abstract</jats:title><jats:p><jats:bold>Finding the few</jats:bold>: Cell‐surface proteins are useful disease biomarkers, but current high‐throughput methods are limited to detecting cells expressing more than several hundred proteins. Enzymatic amplification in microfluidic droplets (see picture) is a high‐throughput method for detection and analysis of cell‐surface biomarkers expressed at very low levels on individual human cells. Droplet optical labels allow concurrent analysis of several samples.<jats:boxed-text content-type="graphic" position="anchor"><jats:graphic xmlns:xlink="http://www.w3.org/1999/xlink" mimetype="image/gif" position="anchor" specific-use="enlarged-web-image" xlink:href="graphic/mcontent.gif"><jats:alt-text>magnified image</jats:alt-text></jats:graphic></jats:boxed-text></jats:p>