Schmidt, Samuel;
Adjobo‐Hermans, Merel J. W.;
Wallbrecher, Rike;
Verdurmen, Wouter P. R.;
Bovée‐Geurts, Petra H. M.;
van Oostrum, Jenny;
Milletti, Francesca;
Enderle, Thilo;
Brock, Roland
Detecting Cytosolic Peptide Delivery with the GFP Complementation Assay in the Low Micromolar Range
You can manage bookmarks using lists, please log in to your user account for this.
Media type:
E-Article
Title:
Detecting Cytosolic Peptide Delivery with the GFP Complementation Assay in the Low Micromolar Range
Contributor:
Schmidt, Samuel;
Adjobo‐Hermans, Merel J. W.;
Wallbrecher, Rike;
Verdurmen, Wouter P. R.;
Bovée‐Geurts, Petra H. M.;
van Oostrum, Jenny;
Milletti, Francesca;
Enderle, Thilo;
Brock, Roland
Published:
Wiley, 2015
Published in:
Angewandte Chemie International Edition, 54 (2015) 50, Seite 15105-15108
Language:
English
DOI:
10.1002/anie.201505913
ISSN:
1433-7851;
1521-3773
Origination:
Footnote:
Description:
AbstractTransfection of cells with a plasmid encoding for the first ten strands of the GFP protein (GFP1‐10) provides the means to detect cytosolic peptide import at low micromolar concentrations. Cytosolic import of the eleventh strand of the GFP protein either by electroporation or by cell‐penetrating peptide‐mediated import leads to formation of the full‐length GFP protein and fluorescence. An increase in sensitivity is achieved through structural modifications of the peptide and the expression of GFP1‐10 as a fusion protein with mCherry.