Media type: E-Article Title: Spin trapping study of reactive oxygen species formation during bopindolol peroxidation Contributor: Kruk, Irena; Michalska, Teresa; Kladna, Aleksandra; Aboul‐Enein, Hassan Y. imprint: Wiley, 2002 Published in: Biopolymers Language: English DOI: 10.1002/bip.10213 ISSN: 0006-3525; 1097-0282 Keywords: Organic Chemistry ; Biomaterials ; Biochemistry ; General Medicine ; Biophysics Origination: Footnote: Description: <jats:title>Abstract</jats:title><jats:p>The generation of singlet molecular oxygen (<jats:sup>1</jats:sup>O<jats:sub>2</jats:sub>) and hydroxyl radicals (HO ·) during peroxidation of bopindolol in the presence of Co(II) ions was studied using electron spin resonance (ESR) and spectrophotometry methods. 2,2,6,6‐Tetramethyl‐4‐piperidone and 5,5‐dimethyl‐1‐pyrroline‐1‐oxide were used as traps. The spectrophotometry determination of <jats:sup>1</jats:sup>O<jats:sub>2</jats:sub> was based on bleaching of <jats:italic>p</jats:italic>‐nitrosodimethylaniline (RNO), which was caused by the product of the reaction of <jats:sup>1</jats:sup>O<jats:sub>2</jats:sub> with imidazole and was followed by monitoring the decrease in optical density at 440 nm. The effect of <jats:sup>1</jats:sup>O<jats:sub>2</jats:sub> quenchers and oxygen free radical scavengers on the ESR signal and the bleaching of RNO was studied. The data presented here give new evidence for generation of the reactive oxygen species during peroxidation of bopindolol. © 2002 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy) 65: 89–94, 2002</jats:p>