Description:
<jats:title>Abstract</jats:title><jats:p>A simple, accurate and precise procedure for the quantitation of itazigrel (a potent lipophilic inhibitor of collagen and arachidonic acid‐induced aggregation being studied for its effects on peripheral vascular disease) from granulated rodent diet is presented. The drug was extracted from rodent diet using methanol + water (80:20) following dissolution of the diet in water. Samples of the supernatant were injected into the HPLC and the eluent was monitored with a fluorescent detector (λ<jats:sub>ex</jats:sub> = 320 and λ<jats:sub>em</jats:sub> = 430) to achieve analytical specificity. Interday coefficients of variation of the calibration curve slope were ±6% on standards between 0 and 1000 μg/g. Potency and homogeneity of the drug spiked diet prepared over a 1 year interval at 70,200 and 600 μg/g was 99.3 ± 2.5%, 100 ± 1.8%, and 101 ± 1.9% of label, respectively. Samples prepared for chromatography were stable for 24 h at 20°C, and drug in diet was stable for 102 days when protected from light and stored at 20°C.</jats:p>