Media type: E-Article Title: Determination of daumone in mouse plasma by HPLC/MS‐MS Contributor: Noh, Keumhan; Park, Jong Hee; Kim, Minkyu; Jung, Mankil; Lee, Hwa Jeong; Kwon, Kwang‐il; Kang, Wonku; Ha, Hunjoo Published: Wiley, 2012 Published in: Biomedical Chromatography, 26 (2012) 2, Seite 152-155 Language: English DOI: 10.1002/bmc.1649 ISSN: 0269-3879; 1099-0801 Keywords: Clinical Biochemistry ; Drug Discovery ; Pharmacology ; Molecular Biology ; General Medicine ; Biochemistry ; Analytical Chemistry Origination: Footnote: Description: <jats:title>ABSTRACT</jats:title><jats:p>Daumone, a pheromone secreted by <jats:italic>Caenorhabditis elegans</jats:italic>, is an essential regulator of chemosensory processes in development and aging. A quantification method using HPLC/MS‐MS was developed for the determination of daumone in mouse plasma. After simple protein precipitation with acetonitrile including methaqualone (an internal standard), the analytes were chromatographed on a reversed‐phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma daumone concentrations following a 5‐week repeated oral administration of daumone in mice. Copyright © 2011 John Wiley & Sons, Ltd.</jats:p>