> Details

Moraes, Ana C. G.; Bertanha, Camila S.; Gimenez, Valéria M. M.; Groppo, Milton; Silva, Márcio L. A.; Cunha, Wilson R.; Januário, Ana H.; Pauletti, Patrícia M.

Development and validation of a high‐performance liquid chromatography method for quantification of egonol and homoegonol in Styrax species

Reference
management

Bookmarks

Remove from
bookmarks

Share this on Whatsapp

Close

Bookmarks



You can manage bookmarks using lists, please log in to your user account for this.
  • Media type: E-Article
  • Title: Development and validation of a high‐performance liquid chromatography method for quantification of egonol and homoegonol in Styrax species
  • Contributor: Moraes, Ana C. G.; Bertanha, Camila S.; Gimenez, Valéria M. M.; Groppo, Milton; Silva, Márcio L. A.; Cunha, Wilson R.; Januário, Ana H.; Pauletti, Patrícia M.
  • imprint: Wiley, 2012
  • Published in: Biomedical Chromatography
  • Language: English
  • DOI: 10.1002/bmc.1744
  • ISSN: 1099-0801; 0269-3879
  • Keywords: Clinical Biochemistry ; Drug Discovery ; Pharmacology ; Molecular Biology ; General Medicine ; Biochemistry ; Analytical Chemistry
  • Origination:
  • Footnote:
  • Description: <jats:title>ABSTRACT</jats:title><jats:p><jats:italic>Styrax camporum</jats:italic> Pohl, known in Brazil as ‘estoraque do campo’ or ‘cuia de brejo’, has been used in the treatment of gastrointestinal diseases. The therapeutic action of <jats:italic>S</jats:italic>. <jats:italic>camporum</jats:italic> has been attributed to the ethyl acetate fraction, although the chemical composition of this fraction has not yet been analyzed. In this study, a high‐performance liquid chromatography photodiode array detection (HPLC‐PAD) method for analysis of Brazilian <jats:italic>Styrax</jats:italic> species has been developed. The compounds egonol (1) and homoegonol (2) were found to be present in all the samples investigated by HPLC. These compounds were isolated by open column chromatography followed by preparative TLC, and were identified by <jats:sup>1</jats:sup>H NMR. Compounds 1 and 2 were thus proposed as phytochemical markers for <jats:italic>Styrax</jats:italic>, owing to their biological properties and presence in other <jats:italic>Styrax</jats:italic> species. The developed method has been validated and successfully applied for quantification of 1 and 2 in <jats:italic>S</jats:italic>. <jats:italic>camporum</jats:italic> dried leaves and crude ethanolic extracts from <jats:italic>S</jats:italic>. <jats:italic>ferrugineus</jats:italic> and <jats:italic>S</jats:italic>. <jats:italic>pohlii</jats:italic> aerial parts. Copyright © 2011 John Wiley &amp; Sons, Ltd.</jats:p>